Trehalose 6-Phosphate Synthase and Trehalose 6-Phosphate Phosphatase from Nicotiana tabacum Function in Trehalose Biosynthesis and Environmental Stress Tolerance of Yeast
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چکیده
منابع مشابه
Trehalose-6-phosphate synthase/phosphatase complex from bakers' yeast: purification of a proteolytically activated form.
A protein of about 800 kDa with trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) activity was purified from bakers' yeast. This TPS/P complex contained 57, 86 and 93 kDa polypeptides. The 86 and 93 kDa polypeptides both appeared to be derived from a polypeptide of at least 115 kDa in the native enzyme. A TPS-activator (a dimer of 58 kDa subunits) was also purifie...
متن کاملInteractions among sites responsible for trehalose 6-phosphate and trehalose-activated glucose 6-phosphate hydrolysis on trehalose phosphatase isolated from Phormia regina.
An enzyme capable of carrying out a trehalose-activated hydrolysis of glucose 6-phosphate has been purified 86-fold from whole body extracts of Phormia regina. By means of a number of criteria, it has been shown that the same enzyme is capable of activity against trehalose-6-P, although hydrolysis of the latter substrate is not activated by trehalose. It appears that the hydrolytic sites are ov...
متن کاملA trehalose-6-phosphate synthase gene from Phellinus igniarius
Trehalose, an important disaccharide in fungal spores, plays important role in protects against many environmental stresses, such as heat and salt. In this study, a gene (PiTPS) encoded trehalose-6-phosphate synthase was isolated from Phellinus igniarius (L.: Fr.) Quél, a basidiomycete fungus belonging to the family Polyporaceae. It contains many bioactive compounds that have been reported to p...
متن کاملDisruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity.
Preparations of the trehalose-6-phosphate synthase/phosphatase complex from Saccharomyces cerevisiae contain three polypeptides with molecular masses 56, 100 and 130 kDa, respectively. Recently, we have cloned the gene for the 56-kDa subunit of this complex (TPS1) and found it to be identical with CIF1, a gene essential for growth on glucose and for the activity of trehalose-6-phosphate synthas...
متن کاملTrehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues.
Axenically grown Arabidopsis thaliana plants were analysed for the occurrence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from Arabidopsis inflorescences. In a variety of organisms, the synthesis of trehalose is catalysed by trehalose-6-phosphate synthase (TPS; EC 2.4.1.15) and trehalose-6-phosphate phosphatase (T...
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ژورنال
عنوان ژورنال: Journal of the Faculty of Agriculture, Kyushu University
سال: 2010
ISSN: 0023-6152
DOI: 10.5109/18840